Sexually dimorphic regulation of estrogen receptor alpha mRNA in the ventromedial hypothalamus of adult whiptail lizards is testosterone dependent.
نویسندگان
چکیده
Female, but not male, whiptail lizards will respond to exogenous estrogen with an increase in estrogen receptor alpha (ER alpha) mRNA in the ventromedial hypothalamus (VMH). The current study tested whether differences in long-term testosterone exposure may be responsible for this sex difference. In the first experiment, female whiptails were gonadectomized and implanted for 6 weeks with either a Silastic capsule containing testosterone or an empty capsule after which the implant was removed. In a second experiment, male whiptail lizards were castrated for 1 week or for 6 weeks. In both experiments animals were then injected with either estradiol benzoate or steroid suspension vehicle and the brains collected 24 h later. Brain mRNA expression was assayed using in situ hybridization. Chronic testosterone treatment reduced estrogen-induced ER alpha mRNA levels (number of grains per cell) in the VMH in females. In contrast, there were no significant effects of time after castration or estrogen treatment on induction of ER alpha mRNA in the VMH in males. The abundance of estrogen-induced ER alpha mRNA in the VMH, therefore, appears to be responsive to testosterone environment in the female but not in the male, suggesting a difference in this species between the sexes in the mechanism of steroid receptor regulation.
منابع مشابه
Effect of long-term castration and long-term androgen treatment on sexually dimorphic estrogen-inducible progesterone receptor mRNA levels in the ventromedial hypothalamus of whiptail lizards.
In whiptail lizards, as in laboratory rodents, females will respond to exogenous estrogen by increasing progesterone receptor (PR) or PR mRNA in the ventromedial hypothalamus (VMH) while males show an attenuated response to the same treatment. In rodents, neonatal hormone manipulations affect the adult expression of this trait; however, few investigators have examined the effects of hormone tre...
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ورودعنوان ژورنال:
- Brain research
دوره 1004 1-2 شماره
صفحات -
تاریخ انتشار 2004